Antibacterial Activity of Leaves and Rhizome of Curcuma xanthorrhiza Essential Oils on Different Distillation Time

Nur Syafiqah Nadiah Mohammad Rafi (1), Irmanida Batubara (2), Anthony Nyangson Steven (3)
(1) Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Malaysia, Indonesia,
(2) Department of Chemistry and Tropical Biopharmaca Research Center, Institut Pertanian Bogor, Indonesia, Indonesia,
(3) Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Malaysia, Indonesia

Abstract

This research aims to determine the antibacterial activity of Curcuma xanthorrhiza leaves and rhizomes essential oil with different distillation time of 2 hours, 4 hours and 6 hours against Escherichia coli and Staphylococcus aureus. The essential oils produced then separated by thin layer chromatography (TLC) with n-hexane: toluene: dichloromethane 1:8:1 as the mobile phase. The result shows that on the first two hours distillation gave the highest yield compared to the next two hours or four hours. Based on TLC chromatograms, the highest content of compounds found at 4-6 hours distillation time. TLC bioautography contact was used for antibacterial activity tested on chromatogram and the clear zone appears on it indicates the spots were active as antibacterial activity against both bacteria with Rf values of 0.35 and 0.49 for rhizomes, rhizome essential oils 4-6 hours distillation time as the most significance active.

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References

Batubara I, Julita I, Darusman LK, Muddathir AM, Mitsunaga T. 2015. Flower bracts of temulawak (Curcuma xanthorrhiza) for skin care: anti-acne and whitening agents. Procedia chemistry 14:216-224
Batubara I, Wahyuni WT, Sutanta M. 2016. Antibacterial activity of zingiberaceae essential oils against Streptococcus mutans and teeth biofilm degradation. IJPBS. 7(4): p111-116
Hamburger MO, & Cordell GA. 1987. A direct bioautographic TLC assay for compounds possessing antibacterial activity. Journal of Natural Products. 50(1): 19-22.
Itokawa H, Hirayama F, Funakoshi K, Takeya K. 1985. Studies on the antitumor bisabolane sesquiterpenoids isolated from Curcuma xanthorrhiza. Chemical and Pharmaceutical Bulletin. 33(8): 3488-3492
Kalor H, & Atun S. 2017. The Antibacterial Activity of Curcuma xanthorrhiza Extract Against Escherichia coli ATCC 11229 and Staphylococcus aureus ATCC 25923. Jurnal Penelitian Saintek. 22(2): 140-147.
Mary HPA, Susheela GK, Jayasree S, Nizzy AM, Rajagopal B, Jeeva S. 2012. Phytochemical characterization and antimicrobial activity of Curcuma xanthorrhiza Roxb. Asian Pacific Journal of Tropical Biomedicine. 2(2) supplement S637-S640
Farag M, Foud I, Ahmed WJ, Mohamed MS, Mohammed MS. 2015. The Role of Natural Products In Drug Discovery And Development. World Journal of Pharmaceutical Research, 4(3).
Oon SF, Nallappan M, Tee TT, Shohaimi S, Kassim NK, Sa’ariwijaya MSF, Cheah YH. 2015. Xanthorrhizol: a review of its pharmacological activities and anticancer properties. Cancer Cell International. 15(1), 100. doi:10.1186/s12935-015-0255-4
Ozaki Y. 1990. Anti-inflammatory effect of Curcuma xanthorrhiza ROXB and its active principles. Chemical and Pharmaceutical Bulletin. 38(4):1045-1048
Rukayadi Y, Hwang JK. 2007. In vitro antimycotic activity of xanthorrhizol isolated from Curcuma xanthorrhiza Roxb against opportunistic filamentous fungi. Phytotherapy Research. 21(5):434-438.
Sok-Lai Hong G-SL, Rahman SNSA, Hamdi OAA, Awang K, Nugroho NA, Malek SN. 2014. Essential oil content of the rhizome of Curcuma purpurascens Bl.(Temu Tis) and its antiproliferative effect on selected human carcinoma cell lines. The Scientific World Journal. 2014(3).
Sylvester WS, Son R, Lew KF, Rukayadi Y. 2015. Antibacterial activity of Java turmeric (Curcuma xanthorrhiza Roxb) extract against Klebsiella pneumoniae isolated from several vegetables. International Food Research Journal. 22(5):1770-1776
Yasni S, Imaizumi K, Sin K, Sugano M, Monaka G, Sidik. 1994. Identification of an active principle in essential oils and hexane soluble fraction of Curcuma xanthorrhiza Roxb showing triglyceride lowering action in rats. Food and Chemical Toxicology. 32:273-378

Authors

Nur Syafiqah Nadiah Mohammad Rafi
Irmanida Batubara
ime@apps.ipb.ac.id (Primary Contact)
Anthony Nyangson Steven
Nadiah Mohammad Rafi, N. S., Batubara, I., & Steven, A. N. (2018). Antibacterial Activity of Leaves and Rhizome of Curcuma xanthorrhiza Essential Oils on Different Distillation Time. Jurnal Jamu Indonesia, 3(3), 116–120. https://doi.org/10.29244/jji.v3i3.69

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