In Vitro Propagation of Temulawak (Curcuma xanthorrhiza Roxb.)
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Temulawak, Curcuma xanthorrhiza, IBA, BAP, In vitro
Abstract
Problems encountered in the development of herbal medicine industry is that most of the raw materials (80%) came from the forest or natural habitats and the rest (20%) of the results of traditional cultivation. The research is focused to get the propagation techniques of temulawak by in vitro using various combinations of IBA and BAP concentration, each consisting of 0, 1, 2, 3, 4 ppm. The purpose of this research is to gain a plant propagation technique of temulawak through the use of IBA and BAP concentration so as to provide a quality seeds. The results showed that the highest shoots obtained at 1 ppm IBA and 3 ppm BAP treatment with a buds height of 15.9 cm. Treatment of 0 ppm IBA and 4 ppm BAP produces the highest buds number by 4 buds. While the highest number of leaves obtained in 2 ppm IBA and 3 ppm BAP treatment with total of 18 leafs. The highest length of leaf obtained in the treatment of 3 ppm IBA and 2 ppm BAP with a length of 14.6 cm. Variable highest number of roots was obtained at treatment of 4 ppm IBA and 1 ppm BAP with root number exceeds 30 pieces with very many hair roots and the highest root length was obtained at treatment of 2 ppm IBA and 0 ppm BAP with a length of 15.6 cm. Based on these results it can be concluded that the IBA and BAP treatment at various concentrations can affect the propagation and growth of temulawak explants.
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References
Anand P, Kunnumakkara AB, Newman RA, Aggarwal BB. 2007. Bioavailability of curcumin: problems and promises. Molecular Pharmaceutics 4:807-18.
Aznam N, Atun S 2016. Pharmacological test of herbal products from temulawak (Curcuma Xanthorhiza) as antihypercholesterol by in vivo. International Journal of Pharmacognosy and Phytochemical Research 8(5):807-811.
Bermawie N, Rahardjo, Wahyuno, Makmun. 2006. Status Teknologi Budidaya dan Pasca Panen Tanaman Kunyit Dan Temulawak Sebagai Penghasil Kurkumin. EDSUS Littro. 2(4):84-99.
BPOM. 2005. Peraturan Kepala Badan Pengawas Makanan tentang Kriteria dan tata laksana Pendaftaran Obat Tradisional, Obat Herbal Terstandar dan Fitofarmaka. BPOM Jakarta.
Devaraj S, Ismail S, Ramanathan S, Yam MF. 2013. In vivo toxicological investigations of standardized ethanolic extract of Curcuma xanthorrhiza Roxb. rhizome. Journal of Natural Product and Plant Resources. 3(1):67-73.
Hendaryono D, Wijayani A. 1994. Teknik Kultur Jaringan: Pengenalan dan Petunjuk Perbanyakan Tanaman Secara Vegetatif-Modern. Penerbit Kanisius. Yogyakarta
Hwang JK, Rukayadi Y. 2006. Challenges and opportunities in applying temulawak (Curcuma xanthorrhiza Roxb.) for industrial oral care products. Prosiding Seminar Nasional Himpunan Kimia Indonesia. Bogor (ID): Departemen Kimia FMIPA IPB dan Himpunan Kimia Indonesia, pp 25-32.
Indah PN, Ermavitalini D. 2013. Induksi kalus daun nyamplung (Calophyllum inophyllum Linn.) pada beberapa kombinasi konsentrasi 6-Benzylaminopurine (BAP) dan 2,4-Dichlorophenoxyacetic Acid (2,4-D). Jurnal Sains dan Seni Pomits. 2(1):E-1 – E-6
Madhulatha P, Anbalagan M, Jayachandaran S, Sakthivel N. 2004. Influence of liquid pulse treatment with growth regulators on In vitro propagation of banana (Musa sp. AAA). Plant Cell, Tissue and Organ Culture. 76:189-192.
Musfiroh I, Muchtaridi M, Muhtadi A, Diantini A, Hasanah AN, Udin LZ, Susilawati Y, Mustarichie R, Kartasasmita RE, Ibrahim S. 2013. Cytotoxicity studies of xanthorrhizol and its mechanism using molecular docking simulation and pharmacophore modelling. Journal of Applied Pharmaceutical Science. 3(06):007-015.
Oon SF, Nallappan M, Tee TT, Shohaimi S, Kassim NK, Sa’ariwijaya MSF, Cheah YH. 2015. Xanthorrhizol: a review of its pharmacological activities and anticancer properties. Cancer Cell International 15:100.
Patma U, Agustina LPP, Siregar L. 2013. Media tanam dan pemberian auksin asam asetat naftalen pada pembibitan aren (Arenga pinnata Merr.). Jurnal Online Agroteknologi. 1(2):1-10
Rabbani MG, Ali MH, Mondal MF. 1996. Effect of BAP and IBA on micropropagation of some banana cultivars. Bangladesh Horticulture. 25(1 dan 2):47-52.
Rahayu S, Hadi WA. 2012. The effect of BAP and thidiazuron on in vitro growth of java turmeric (Curcuma xanthorrhiza Roxb.). ARPN Journal of Agricultural and Biological Sciences. 7(10):820-824.
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