Evaluasi Faktor Yang Mempengaruhi Ekstraksi Rimpang Temu Ireng Berdasarkan Aktivitas Penghambatan α-Glukosidase
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alfa-glukosidase, Ektraksi, Rancangan faktorial fraksional, Temu ireng
Abstract
Terdapat beberapa faktor yang dapat digunakan dalam ekstraksi rimpang temu ireng yang memiliki aktivitas farmakologi. Penelitian ini bertujuan untuk mengidentifikasi faktor pH, konsentrasi etanol, suhu, waktu, dan rasio cairan-padatan dalam proses ekstraksi rimpang yang memberikan kontribusi signifikan sebagai inhibitor enzim a-glukosidase. Rancangan optimasi dilakukan dengan menggunakan faktoral fraksional 2(5-1). Urutan hasil dari ekstrak yang paling berkontribusi sebagai inhibitor enzim a-glukosidase: konsentrasi etanol > rasio cairan terhadap padatan > suhu > waktu > pH. Diantara faktor yang dianalisis menunjukkan bahwa konsentrasi etanol dan interaksi antara pH dan konsentrasi etanol merupakan faktor yang signifikan sebagai inhibitor enzim a-glukosidase. Dengan demikian, rimpang temu ireng memiliki potensi sebagai anti-hiperglikemia alami.
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