Optimization of Black Orchid (Coelogyne pandurata Lindl.) Extraction Using Simplex-Centroid Design for Total Phenolics, Flavonoids, and Antioxidants
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Antioxidant compounds, Black orchid, Extraction design
Abstract
Coelogyne pandurata Lindl., a black orchid endemic to Kalimantan, contains valuable antioxidant compounds. This study optimized its extraction using a Simplex-Centroid Mixture Design with ethanol, methanol, and acetic acid as solvents. Responses evaluated were total phenolic content (TPC), total flavonoid content (TFC), and antioxidant capacity (DPPH). The highest TPC was obtained with 100% ethanol (2.52 mg GAE/g), TFC with equal parts of all solvents (6.41 mg QE/g), and antioxidant activity with 100% methanol (10.33 µmol TE/g). Ethanol yielded the optimal extraction condition, with verification values of 2.27 mg GAE/g (TPC), 3.42 mg QE/g (TFC), and 10.22 µmol TE/g (DPPH), and a desirability of 0.739. Although regression models were not statistically significant (p > 0.05), the results provide preliminary insights into solvent efficiency for extracting bioactive compounds. Further studies with expanded experimental designs and validation are required to enhance model accuracy and extraction reliability.
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References
Arista, R. A., & Nurcholis, W. (2024). Secondary metabolites of Java cardamom leaves extract function under shading and nitrogen doses. Revista Brasileira de Engenharia Agrícola e Ambiental, 28, e274639. https://doi.org/10.1590/1807-1929/agriambi.v28n1e274639
Azahar, N. F., Abd Gani, S. S., Zaidan, U. H., Bawon, P., & Halmi, M. I. E. (2020). Optimization of the antioxidant activities of mixtures of Melastomataceae leaves species using a simplex-centroid design and their anti-collagenase and elastase properties. Applied Sciences, 10(19), 7002. https://doi.org/10.3390/app10197002
Cavalcanti, V. P., Aazza, S., Bertolucci, S. K. V., Rocha, J. P. M., Coelho, A. D., Oliveira, A. J. M., Mendes, L. C., Pereira, M. M. A., Morais, L. C., Forim, M. R., Pasqual, M., & Dória, J. (2021). Solvent mixture optimization in the extraction of bioactive compounds and antioxidant activities from garlic (Allium sativum L.). Molecules, 26(19), 6026. https://doi.org/10.3390/molecules26196026
El Mannoubi, I. (2023). Impact of different solvents on extraction yield, phenolic composition, in vitro antioxidant and antibacterial activities of deseeded Opuntia stricta fruit. Journal of Umm Al-Qura University for Applied Sciences, 9(2), 176–184. https://doi.org/10.1007/s43994-023-00031-y
Heriansyah, P., Aziz, S. A., Sukma, D., & Nurcholis, W. (2025a). Antioxidant capacity of Coelogyne pandurata extracts at different phenological phases. Revista Brasileira de Engenharia Agrícola e Ambiental, 29, e279352. https://doi.org/10.1590/1807-1929/agriambi.v29n2e279352
Heriansyah, P., Aziz, S. A., Sukma, D., & Nurcholis, W. (2025b). Enhancing total phenolic, flavonoid, and antioxidant capacity in black orchid (Coelogyne pandurata Lindl.) with paclobutrazol application. BIO Web of Conferences, 171, 01007. https://doi.org/10.1051/bioconf/202517101007
Jdaini, K., Alla, F., Mansouri, F., Parmar, A., & Elhoumaizi, M. A. (2023). Optimizing the extraction of phenolic antioxidants from date palm fruit by simplex-centroid solvent mixture design. Heliyon, 9(1), e12738. https://doi.org/10.1016/j.heliyon.2022.e12738
Navarro-Cortez, R. O., Santiago-Saenz, Y. O., López-Palestina, C. U., Gutiérrez-Tlahque, J., & Piloni-Martini, J. (2023). Application of a simplex–centroid mixture design to evaluate the phenolic compound content and antioxidant potential of plants grown in Mexico. Foods, 12(18), 3479. https://doi.org/10.3390/foods12183479
Nurcholis, W., Alfadzrin, R., Izzati, N., Arianti, R., Vinnai, B. Á., Sabri, F., Kristóf, E., & Artika, I. M. (2022). Effects of methods and durations of extraction on total flavonoid and phenolic contents and antioxidant activity of Java cardamom (Amomum compactum Soland. ex Maton) fruit. Plants, 11(17), 2221. https://doi.org/10.3390/plants11172221
Routray, W., & Orsat, V. (2012). Microwave-assisted extraction of flavonoids: A review. Food and Bioprocess Technology, 5(2), 409–424. https://doi.org/10.1007/s11947-011-0573-z
Soussi, M., Fadil, M., Yaagoubi, W. A., Benjelloun, M., & El Ghadraoui, L. (2022). Simultaneous optimization of phenolic compounds and antioxidant abilities of Moroccan Pimpinella anisum extracts using mixture design methodology. Processes, 10(12), 2580. https://doi.org/10.3390/pr10122580
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