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Rice bran, a rice by-product yielded by the milling process, has recently been recognized as a potential source of natural active constituents. Black rice bran has the highest antioxidant capacity compared to white and brown rice bran. It is correlated with the contribution of phenolic compounds in rice bran. The purpose of this study was to determine the total phenolic content in black rice (Oryza sativa L. indica) bran ethanolic extract from two different regions in Java, Indonesia, using Folin-Ciocalteu Reagent (FCR). Black rice bran samples used in this study were planted in Ciletuh Geopark Sukabumi, West Java (BRBE1), and Karanganyar Regency, Central Java (BRBE2). The parameters observed were total phenolic content, and the result was statistically tested with an unpaired t-test by GraphPad Prism 8.3.0 application. The samples contained 175.48 mg GAE/g extract in BRBE1 and 174.39 mg GAE/g extract in BRBE2. There was no significant difference based on these results. It can be concluded that both samples contained phenolic compounds and statistically, there was no significant difference.


ethanolic extract total phenolic content Folin-Ciocalteu Reagent black rice bran

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Vardhani, A., Anggraini, N. B., Jufri, M. ., Purwaningsih, E. ., & Perdanastuti, H. S. . (2024). Total Phenolic Content in Black Rice (Oryza sativa L. indica) Bran Ethanolic Extract from Two Different Regions in Java, Indonesia. Jurnal Jamu Indonesia, 9(1), 3–7.


  1. Akman, S., Demirata-Ozturk, B., & Tokman, N. (2007). Atomic Absorption Spectroscopy. In Food Toxicants Analysis: Techniques, Strategies and Developments (pp. 637–665). Elsevier.
  2. Ardiansyah, N., Nugraha, K. S. A., & Ikhram, R. (2019). Geodiversity of Ciletuh-Palabuhanratu UNESCO Global Geopark, Sukabumi, West Java. Berita Sedimentologi, 44(44), 11–18.
  3. Chaudhary, R. C. (2003). Speciality rices of the world: Effect of WTO and IPR on its production trend and marketing. Journal of Food Agriculture & Environment, 1(2), 34–41.
  4. Chen, M. H., & Bergman, C. J. (2005). A rapid procedure for analysing rice bran tocopherol, tocotrienol and γ-oryzanol contents. Journal of Food Composition and Analysis, 18(2–3), 139–151.
  5. Departemen Kesehatan Republik Indonesia. (1995). Farmakope Indonesia V. Departemen Kesehatan Republik Indonesia.
  6. Departemen Kesehatan Republik Indonesia. (1980). Materia medika IV. Departemen Kesehatan Republik Indonesia.
  7. Dou, F., Soriano, J., Tabien, R. E., & Chen, K. (2016). Soil Texture and Cultivar Effects on Rice (Oryza sativa, L.) Grain Yield, Yield Components and Water Productivity in Three Water Regimes. PLoS ONE, 11(3), e0150549.
  8. Farasat, M., Khavari-Nejad, R.-A., Nabavi, S. M. B., & Namjooyan, F. (2013). Antioxidant Properties of two Edible Green Seaweeds From Northern Coasts of the Persian Gulf. Jundishapur Journal of Natural Pharmaceutical Products, 8(1), 47–52.
  9. Galanakis, C. M., Goulas, V., Tsakona, S., Manganaris, G. A., & Gekas, V. (2013). A knowledge base for the recovery of natural phenols with different solvents. International Journal of Food Properties, 16(2), 382–396.
  10. Goufo, P., & Trindade, H. (2014). Rice antioxidants: Phenolic acids, flavonoids, anthocyanins, proanthocyanidins, tocopherols, tocotrienols, c-oryzanol, and phytic acid. Food Science and Nutrition, 2(2), 75–104.
  11. Hudz, N., Yezerska, O., Shanaida, M., Sedláčková, V. H., & Wieczorek, P. P. (2019). Application of the Folin-Ciocalteu method to the evaluation of Salvia sclarea extracts. Pharmacia, 66(4), 209–215.
  12. Jung, T. D., Shin, G. H., Kim, J. M., Choi, S. Il, Lee, J. H., Lee, S. J., Park, S. J., Woo, K. S., Oh, S. K., & Lee, O. H. (2017). Comparative analysis of γ-oryzanol, β-glucan, total phenolic content and antioxidant activity in fermented rice bran of different varieties. Nutrients, 9(6), 571.
  13. Laokuldilok, T., Shoemaker, C. F., Jongkaewwattana, S., & Tulyathan, V. (2011). Antioxidants and antioxidant activity of several pigmented rice brans. Journal of Agricultural and Food Chemistry, 59(1), 193–199.
  14. Nam, S. H., Choi, S. P., Kang, M. Y., Koh, H. J., Kozukue, N., & Friedman, M. (2006). Antioxidative activities of bran extracts from twenty one pigmented rice cultivars. Food Chemistry, 94(4), 613–620.
  15. Parrado, J., Miramontes, E., Jover, M., Gutierrez, J. F., Collantes de Terán, L., & Bautista, J. (2006). Preparation of a rice bran enzymatic extract with potential use as functional food. Food Chemistry, 98(4), 742–748.
  16. Putra, A. S., Setyowati, N., & Ani, S. W. (2017). Efisiensi usahatani padi beras hitam di Kabupaten Karanganyar. SEPA: Jurnal Sosial Ekonomi Pertanian Dan Agribisnis, 13(1), 48.
  17. Raharjo, S., Purwandari, F. A., Hastuti, P., & Olsen, K. (2019). Stabilization of Black Rice (Oryza Sativa, L. Indica) Anthocyanins Using Plant Extracts for Copigmentation and Maltodextrin for Encapsulation. Journal of Food Science, 84(7), 1712–1720.
  18. Rini, N. K., & Sofiani, V. (2018). Pengaruh preferensi dan faktor konsumen yang mempengaruhi manajemen pemasaran dari padi hitam di Geopark Ciletuh Pelabuhan Ratu. Ummi, 12(3), 47–52.
  19. Ruen-Ngam, D., Thawai, C., Nokkoul, R., & Sukonthamut, S. (2014). Gamma-Oryzanol Extraction from Upland Rice Bran. International Journal of Bioscience, Biochemistry and Bioinformatics, 4(4), 252–255.
  20. Sanders, E. R. (2012). Aseptic laboratory techniques: Plating methods. Journal of Visualized Experiments, 63, 1–18.
  21. Sanusan, S., Polthanee, A., Audebert, A., Seripong, S., & Mouret, J. C. (2010). Growth and yield of rice (Oryza sativa L.) as affected by cultivars, seeding depth and water deficits at vegetative stage. Asian Journal of Plant Sciences, 9(1), 36–43.
  22. Shannon, E., Jaiswal, A. K., & Abu-Ghannam, N. (2018). Polyphenolic content and antioxidant capacity of white, green, black, and herbal teas: A kinetic study. Food Research, 2(1), 1–11.
  23. Singh, N., Singh, N., Tripathy, S. S., Soni, D., Singh, K., & Gupta, P. K. (2013). Evaluation of purity with its uncertainty value in high purity lead stick by conventional and electro-gravimetric methods. Chemistry Central Journal, 7(1), 1–10.
  24. Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999). [14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology, 299, 152–178.
  25. Sukrasno, S., Tuty, S., & Fidrianny, I. (2017). Antioxidant evaluation and phytochemical content of various rice bran extracts of three varieties rice from Semarang, Central Java, Indonesia. Asian Journal of Pharmaceutical and Clinical Research, 10(6), 377–382.
  26. Tjitrosoepomo, G. (2005). Morfologi Tumbuhan. UGM Press.
  27. Trinovita, E., Saputri, F. C., & Mun’im, A. (2018). Potential gastroprotective activity of rice bran (Oryza sativa L.) extracted by ionic liquid-microwave-assisted extraction against ethanol-induced acute gastric ulcers in rat model. Scientia Pharmaceutica, 86(3), 35.
  28. UNESCO. (2017). Ciletuh – Pelabuhan Ratu UNESCO global geopark. Retrieved October 15, 2022, from
  29. Vardhani, A., Jufri, M., & Purwaningsih, E. (2020). Potency of γ-oryzanol-rich black rice bran (Oryza Sativa L. Indica) extract for tyrosinase inhibition. International Journal of Pharmacy and Pharmaceutical Sciences, 90–93.
  30. Zaky, A. A., Abd El-Aty, A. M., Ma, A., & Jia, Y. (2022). An overview on antioxidant peptides from rice bran proteins: extraction, identification, and applications. In Critical Reviews in Food Science and Nutrition (Vol. 62, Issue 5, pp. 1350–1362). Taylor & Francis.