Ekstrak Metanol Rhizopora apiculata Sebagai Biosensor Penghambatan Aktivitas Tirosinase Menggunakan Elektrode Pasta Karbon Termodifikasi Ferosena Dan Zeolit
Article Sidebar
-
Elektrode pasta karbon, Ferosena, Inhibitor tirosinase, Rhizopora apiculata, Zeolit
Abstract
Ekstrak metanol tanaman Rhizopora apiculata telah dibuat untuk biosensor inhibitor enzim tirosinase menggunakan elektrode pasta karbon dengan termodifikasi zeolit dan mediator ferosena. Enzim tirosinase merupakan enzim yang mengkatalisis 2 tahap reaksi biosintesis melanin pada mamalia dan berperan dalam reaksi pencokelatan enzimatis. Enzim diimobilisasi pada permukaan transduser untuk menjaga aktivitas enzim. Aktivitas tirosinase diukur menggunakan substrat L-DOPA. Kondisi optimum aktivitas tirosinase berdasarkan pengoptimalan respon adalah tirosinase 50 U/mL dan zeolit 150 mg/mL dalam larutan penyangga fosfat. Penggunaan mediator ferosena dan material penyangga zeolit meningkatkan aktivitas tirosinase. Nilai KMapp dan Imaks app tirosinase ditentukan dengan metode Lineweaver-Burk. Nilai KM app dan Imaks app sebesar 6.04 mM dan sebesar 6.42 mM. Biosensor menggunakan zeolit sebagai matriks imobilisasi dan ferosensa sebagai mediator mempunyai stabilitas respon sampai 2 jam, dengan penurunan aktivitas sebesar 19%.
Full text article
References
Abdullah. 2011. Potensi Bakau Rhizophora apiculata BL sebagai inhibitor tirosinase dan antioksidan [tesis]. Bogor (ID): Sekolah Pascasarjana, Institut Pertanian Bogor.
Arif Z. 2011. Karakterisasi dan modifikasi zeolit alam sebagai bahan media pendeteksi studi kasus: kromium heksavalen [tesis]. Bogor (ID): Sekolah Pascasarjana, Institut Pertanian Bogor.
Arnaut L, Formosinho S, Burrows H. 2007. Chemical Kinetics from Molecular Structure to Chemical Reactivity. Amsterdam (ND):Elsevier.
Arung ET, Shimizu K, Kondo R. 2006. Inhibitory effect of artocarpanone from Artocarpus heterophyllus on melanin biosynthesis. Journal of Biology. 29:1966-1969.
Astruc D. 2016. Why is ferrocene is so exceptional?. European Journal of Inorganic Chemistry. 0000: 1-25. DOI: 10.1002/ejic.201600983.
Batubara I, Darusman LK, Mitsunaga T, Rahminiwati M, Djauhari E. 2010. Potency of Indonesia medicinal plants as tyrosinase inhibitor andantioxidants agent. Journal of Biological Science. 10:138-144.
Bisswanger H. 2008. Enzyme Kinetics Prinsiples and Methods. Weinheim (DE): Wiley-VCH.
Chang TS. 2009. An update review of tyrosinase inhibitors. Journal of Molecular Sciences. 10:2440-2473. doi:10.3390/ijms10062440.
Eslami M, Zare H R, Namazian M. 2012. Thermodynamic parameters of electrochemical oxidation of l-DOPA: Experimental and Theoretical Studies. Journal of Physical Chemistry. B 41:116. doi: 10.1021/jp3054229.
Hartanti L, Setiawan KH. 2009. Inhibitory of some synthetic cinnamic acid derivates towards tyrosinase enzyme. Indonesian Journal of Chemistry. 9(1):158-168.
Heraldy E, His Yam SW, Sulistiono. 2003. Characterization and activation zeolite from ponorogo. Indonesian Journal of chemistry. 3 (2): 91-97.
Liu S, Jiuhong Y, Huangxian J. 2003. Renewable phenol biosensor based on a tyrosinase-colloidal gold modified carbon paste electrode. Journal of Electroanalytical Chemistry. 540: 61-67.
Kim MA, Yoong-Lee W. 2002. Amperometric phenol biosensor based on sol–gel silicate/Nafion composite film. Analytica Chimica Acta. 479:43–150. doi:10.1016/S0003-2670(02)01538-6.
Saiapina OY, Pyeshkova VM, Soldatkin OO, Melnik VG, Akata Kurç B, Walcarius A, Dzyadevych SV, Jaffrezic-Renault N. 2011. Conductometric enzyme biosensors based on natural zeolit clinoptilolite for urea determination. Materials and Science Engineering C. 31:1490-1497. doi:10.1016/j.msec.2011.06.003
Serra PA. 2010. Biosensor. Croatia (HR): Intech.
Sheng, W, Xua, T, Maa H, Wanga X, Li Q, Li J. 2009. Development of an indirect competitive enzyme linked immunosorbent assay for detection of danofloxacin Residues in Beef, Chicken and Pork Meats. Journal of Food And Agricultural Immunology. 20:35-47
Trivadila. 2011. Biosensor antioksidan menggunakan superoksida dismutase Deinococcus radiodurans diimobilisasi pada permukaan elektrode pasta karbon dan parameter kinetikanya. Tesis. Bogor (ID): Sekolah Pascasarjana, Institut Pertanian Bogor.
Wang B, Zheng J, Hae Y, Sheng Q. 2013. A sandwich-type phenolic biosensor based on tyrosinase embeddinginto single-wall carbon nanotubes and polyaniline nanocomposites. Sensors and Actuators B: Chemical. 186 :417– 422.
Wang X, ChenL, Xia S, Zhu Z, Zhao J, Chovelomad J. 2006. Tyrosinase biosensor based on interdigitated electrodes for herbicides determination. Journal of Electrochemistry Sciences. 1:55-61.
Zhang Q, Qu Y, Zhou J, Zhang X, Zhou H, Ma Q, Li X. 2011. Optimization of 2,3-dihydroxybiphenyl 1,2-dioxygenase expression and its application for biosensor. Bioresource Technology. 102:10553-10560.
Authors
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. Copyright @2017. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted non-commercial used, distribution and reproduction in any medium