Heme Polymerization Inhibitory Activity And Phytochemical Screening Of Ethyl Acetate Fraction In Manuran (Coptosapelta tomentosa Valeton ex K. Heyne) Stem
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Hemellpolymerization, Coptosapelta tomentosa Valeton ex K. Heyne, IC50
Abstract
The native Indonesian plant that is empirically used as an antimalarial agent is manuran (Coptosapelta tomentosa Valeton ex K. Heyne). This study aims to determine chemical compound and heme polymerization inhibitory activity of ethyl acetate fraction of C. Tomentosa stem based on IC50 value. The method identification of chemical compound used tube test, and the method of heme polymerization inhibitory activity was Basilico through in vitro method. The results of chemical compound identification of the ethyl acetate fraction of C. Tomentosa showed the presence of flavonoids, terpenoids, saponins, tannins, and anthraquinones. The average percentages of heme polymerization inhibitory activity of ethyl acetate fraction of C. Tomentosa stem from concentration 20; 10; 5; 2.5; 1.25; 0.625; 0.3125 mg / mL were 98.507; 97,872; 96,407; 93,560; 88,419; 80,680; and 45.467%.The averages of IC50 of ethyl acetate fraction and chloroquine diphosphate were 0.24 ± 0.018 mg/mL and 0.214 ± 0.012 mg/mL. This shows that the ethyl acetate fraction of C. Tomentosa stem has heme polymerization inhibitory activity. The result of the independent sample t-Test obtained the significance value of 0.111 (p more than 0.05) that there was no significant difference. It means that the ethyl acetate fraction of C. Tomentosa stem has heme polymerization inhibitory activity as well as chloroquine diphosphate. This suggests the potentiation of the methyl acetate fraction of the stem C. Tomentosa as anti-malarial.
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References
Arnida & Lingga, 2016, Aktivitas Penghambatan Polimerisasi Hem Fraksi n-Butanol Akar Manuran (Coptosapelta Tomentosa Valeton Ex K. Heyne) Asal Kotabaru Kalimantan Selatan, Unpublish
Arnida, Eka Rahmawaty Sahi, Sutomo, Fadlillaturrahma, 2017, Aktivitas Penghambatan Polimerisasi Hem Ekstrak Etanol Batang Manuran (Coptosapelta tomentosa Valeton Ex K.Heyne) Asal Kotabaru Kalimantan Selatan. Prosiding Seminar Nasional Kefarmasian dan Publiksi Ilmia, Perkembangan Terapi Obat Herbal Pada penyakit Degenaratif Banjarmasin 30 September 2017, ISBN 978-602- 50258-0-8.
Arnida. 2015. Isolasi dan Uji Aktivitas Antiplasmodium In Vitro Senyawa Aktif dari Umbi Hati Tanah (Angiopteris evecta). Disertasi, Program Pasca Sarjana Program Studi Ilmu Farmasi, Universitas Gajah Mada, Yogyakarta.
Azhari A.H. 2016. Isolasi dan Identifikasi Senyawa Antioksidan dari Buah Kasuri (Mangifera casturi Kosterm.)Skripsi. Program Studi Farmasi, Universitas Lambung Mangkurat, Banjarbaru.
Baelsmans, R., E. Deharo, V. Munoz, M. Sauvain & H. Ginsburg. 2000. Experimental Conditions for Testing the Inhibitory Activity of Chloroquine on the Formation of ß-Hematin, Experimental Parasitology. 42: 55-60.
Basilico, N., E. Pagani, D. Monti, P. Olliaro, & D. Taramelli. 1998. A Microtitre-Based Method for Measuring the Haem Polymerization Inhibitory Activity (HPIA) of Antimalarial Drugs. Journal of Antimicrobial Chemotherapy.42: 55–60.
Dinkes Kabupaten Banjar. 2012. Laporan Tahunan Bidang Kesehatan Keluarga Tahun 2012. Seksi KIA. Kabupaten Banjar.
Eddy, S. & R.S. Tambunan. 2007. Isolasi dan Identifikasi Senyawa Golongan Flavonoid dalam Fraksi n-Butanol dari Ekstrak Metanol Daun Sidaguri. Jurnal Biologi Farmasi
Gandjar, I. G. & Rohman, A. 2013.Kimia Farmasi Analisis. Pustaka Pelajar, Yogyakarta.
Hakim, L. 2011. Malaria : Epidemiologi dan Diagnosis. Aspirator. 3 : 107-116.
Purwanto. 2011. Isolasi dan Identifikasi Senyawa Penghambat Polimerisasi Hemdari Fungi Endofit Tanaman Artemisia annua L. Tesis. Universitas Gadjah Mada, Yogyakarta.
Syamsudin, Supargiyono, S. Wahyuono, P. Simanjuntak, & Mustofa. 2013. Heme Polymerization Inhibitory Activities of Xanthone from G. Parvifolia (Miq) Miq Stem Bark as An Antimalarial. Asian Journal of Chemistry. 25: 1-4.
Tanaya, V., R. Retnowati & Suratmo. 2015. Fraksi Semi Polar Dari Daun Mangga Kasturi (Mangifera casturi Kosterm). Kimia Student Journal.1: 778-784.
Tiwari, Prashant., B. Kumar., M. Kaur., G. Kaur, & H. Kaur. 2011. Phytochemical Screening and Extraction: A Review. International Pharmaceutica Scienca. 1: 98-106.
Wahyono, Pudjono & P. Widyati. 2010. Uji Aktivitas Senyawa AntiPlasmodium dari Fungi Endofit Tanaman Artemsia annua L. Majalah Farmasi Indonesia. 21: 230-235.
Wijaya, J., J. Salenussa& J. Marantika. 2013. Potensi Ekstrak Metanol Batang Kapur (Harmsiopanax aculeatus Harms) sebagai Obat Antimalaria. Jurnal Kimia. 1-9.
Wijono, S.H. 2003. Isolasi dan Identifikasi Flavonoid pada Daun Katu (Sauropus adrogynus (L.) Merr). Makara, Sains. 2: 52-64.
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