Anticholesterolemic Evaluation of Physalis angulata L. Ethanolic Extract Using In Vitro, GC-MS, and Docking Approaches
Abstract
Physalis angulata L. is traditionally used as a medicinal plant and contains various pharmacologically active metabolites. This study evaluated the anticholesterolemic potential of P. angulata ethanolic extract using in vitro assays, gas chromatography–mass spectrometry (GC-MS), and molecular docking. The extract obtained by remaceration contained flavonoids, phenolics, tannins, saponins, and steroids/triterpenoids. Thin-layer chromatography indicated the presence of terpenoids, flavonoids, essential oils, and saponins. Quantitative analysis showed flavonoid, phenolic, tannin, and sterol contents of 34.86 ± 1.11 mg RE/g, 5.14 ± 0.15 mg GAE/g, 0.99 ± 0.01%, and 0.072 ± 0.0004 mg CE/g, respectively. Antioxidant IC₅₀ values were 209.55 ± 9.10 ppm for DPPH and 220.18 ± 4.23 ppm for FRAP. The Liebermann–Burchard assay showed cholesterol inhibition with an IC₅₀ of 96.11 ppm. GC-MS tentatively identified several compounds, while molecular docking suggested favora
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