Specific and Non-Specific Safety Testing of Red Gedi Leaf Ethanol Extract (Abelmoschus manihot L. Medik)

Joni Tandi(1), Yuliet Susanto(2), Tien Wahyu Handayani(3), Magfirah Magfirah(4), Muthmainah Tuldjanah(5)
(1) Department of Pharmacy, College of Pharmaceutical Sciences Pelita Mas, Palu Central Sulawesi, 94118,
(2) Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Tadulako University, Palu, Central Sulawesi, 94118,
(3) Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Tadulako University, Palu, Central Sulawesi, 94118,
(4) Department of Pharmacy, College of Pharmaceutical Sciences Pelita Mas, Palu Central Sulawesi, 94118,
(5) Department of Pharmacy, College of Pharmaceutical Sciences Pelita Mas, Palu Central Sulawesi, 94118

Abstract

Red gedi leaves (Abelmoschus manihot (L.) Medik) are traditionally used for their antidiabetic, antioxidant, and hepatoprotective properties. This study aimed to characterize the specific and non-specific quality parameters of red gedi leaf ethanol extract based on Herbal Pharmacopoeia standards. Extraction was performed by maceration using 96% ethanol, yielding 6.72%. Phytochemical screening confirmed the presence of flavonoids, saponins, tannins, quinones, and steroids, with a high total flavonoid content of 56.97 ± 3.75 mg/g. Non-specific parameters, including water content, drying loss, ash values, microbial contamination, and heavy metal levels (Pb and Cd), complied with the required standards. Organoleptic evaluation showed a viscous extract with brownish color, slightly astringent taste, and characteristic odor. These results indicate that red gedi leaf ethanol extract meets Herbal Pharmacopoeia quality requirements and has potential for development as a standardized antioxidant herbal product.

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References

Depkes RI. (2000). General standard parameters of medicinal plant extracts. Department of Health of the Republic of Indonesia.

Diao, Z., Yu, H., Wu, Y., Sun, Y., Tang, H., Wang, M., Li, N., Ge, H., Sun, J., & Gu, H. F. (2024). Identification of the main flavonoids of Abelmoschus manihot (L.) Medik and their metabolites in the treatment of diabetic nephropathy. Frontiers in Pharmacology, 14, 1290868. https://doi.org/10.3389/fphar.2023.1290868

Handayani, T. W., Mallisa, M. P., Tandi, J., & Yuliet, Y. (2024). Effect of red gedi leaf extract (Abelmoschus manihot (L.) Medik) on total cholesterol of hypercholesterolemic diabetic male rats. Journal Pharmasci (Journal of Pharmacy and Science), Special Issue, 127–132. https://doi.org/10.53342/pharmasci.vi.428

Imansyah, M. Z., & Alam, G. (2021). Physical quality standardization of ethanol extract of green bitter melon leaves (Momordica carantia L.). Yamasi Makassar Health Journal, 5(2), 121–127.

Kementerian Kesehatan Republik Indonesia. (2022). Supplement I: Indonesian herbal pharmacopoeia (2nd ed.). Ministry of Health of the Republic of Indonesia.

Kumontoy, G. D., Deeng, D., & Mulianti, T. (2023). Utilization of herbal plants as traditional medicine for public health in Guaan Village, Mooat District, East Bolaang Mongondow Regency. Holistik: Journal of Social and Culture, 16(3), 1–16.

Mangalu, M. A., Simbala, H. E. I., & Suoth, E. J. (2022). Standardization of specific parameters of yaki areca nut (Areca vestiaria) extract. Pharmacy Medical Journal, 5(1), 20. https://doi.org/10.35799/pmj.v5i1.41611

Marpaung, M. P., & Septiyani, A. (2020). Determination of specific and non-specific parameters of ethanol condensed extract of yellow root stem (Fibraurea chloroleuca Miers). Journal of Pharmacopolium, 3(2), 58–67. https://doi.org/10.36465/jop.v3i2.622

Mutmainnah, T., Tadjio, Y. K., & Tandi, J. (2018). Nephroprotective effect of red gedi leaf extract on creatinine and urea levels in ethylene glycol-induced male rats. Pharmacology Journal of Pharmacy, 15(2), 160–167.

Qiu, Y., Ai, P. F., Song, J. J., Liu, C., & Li, Z. W. (2017). Total flavonoid extract from Abelmoschus manihot (L.) Medik flowers attenuates D-galactose-induced oxidative stress in mouse liver through the Nrf2 pathway. Journal of Medicinal Food, 20(6), 557–567. https://doi.org/10.1089/jmf.2016.3870

Sobari, E., Ramadhan, M. G., & Destiana, I. D. (2022). Determining the yield value in the mulberry leaf extraction process (Morus alba L.) using different solvents. Scientific Journal of Engineering Science and Technology, 4, 36–41. https://doi.org/10.31962/jiitr.v4i1.66

Sun, Z., Liu, W., Zhang, S., Tian, S., & Aikemu, A. (2024). Optimization of flavonoid extraction from Abelmoschus manihot flowers using ultrasonic techniques and biological activity assessment. Molecules, 29(11), 2610. https://doi.org/10.3390/molecules29112610

Tandi, J. (2018). Analysis of red gedi leaves (Abelmoschus manihot (L.) Medik) as a medicine for diabetes mellitus. EGC.

Tandi, J., H., M., Yuliet, & Yusriadi. (2016). Effectiveness of red gedi leaf extract on blood glucose, malondialdehyde, 8-hydroxy-deoxyguanosine, and insulin levels in diabetic rats. Journal of Tropical Pharmacy and Chemistry, 3(4), 264–276. https://doi.org/10.25026/jtpc.v3i4.114

Tandi, J., Roem, M., & Yuliet, Y. (2017). Nephroprotective effect of combination of red gedi leaf extract and cat whiskers leaf extract on ethylene glycol-induced mice. Journal of Tropical Pharmacy and Chemistry, 4(1), 27–34. https://doi.org/10.25026/jtpc.v4i1.129

Tandi, J., Handayani, T. W., Aril, M., & Polakitan, G. L. (2025). Education on the use of red gedi to overcome diabetes complications. Prima Abdika: Journal of Community Service, 5(1), 147–155. https://doi.org/10.37478/abdika.v5i1

Wardani, T. S., & Setianto, R. (2023). Standarisasi bahan obat alam. Pustaka Baru Press.

Authors

Joni Tandi
jonitandi757@yahoo.com (Primary Contact)
Yuliet Susanto
Tien Wahyu Handayani
Magfirah Magfirah
Muthmainah Tuldjanah
Tandi, J., Susanto, . Y., Handayani, T. W., Magfirah, M., & Tuldjanah, M. (2026). Specific and Non-Specific Safety Testing of Red Gedi Leaf Ethanol Extract (Abelmoschus manihot L. Medik). Jurnal Jamu Indonesia, 11(1), 58-65. https://doi.org/10.29244/jji.v11i1.417

Article Details

How to Cite

Tandi, J., Susanto, . Y., Handayani, T. W., Magfirah, M., & Tuldjanah, M. (2026). Specific and Non-Specific Safety Testing of Red Gedi Leaf Ethanol Extract (Abelmoschus manihot L. Medik). Jurnal Jamu Indonesia, 11(1), 58-65. https://doi.org/10.29244/jji.v11i1.417

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