Penentuan Derajat Toksisitas Akut Ekstrak Air Buah Pepaya (Carica papaya L.) Muda Pada Mencit Menggunakan Purposed New Recommended Method
Article Sidebar
-
Unripe Carica papaya L. fruit aqueous extract, degree of acute toxicity, LD50
Abstract
Papaya (Carica papaya L.) fruit was proven scientifically in having medicinal effects: such as gastroprotective, laxative, galactagogues, and antibacterial effect. Unripe papaya fruit contains saponin, alkaloid, tannin, flavonoid, triterpenoid, and quinone which may reveal toxicities as adverse effect when it consumed in high dose. The aim of this study is to determine the acute toxicity degree of unripe Carica papaya L. fruit aqueous extract in mice using proposed new recommended method. The acute toxicity degree conducted by determination of lethal dose50 (LD50) using new recommended method to 12 female mice. The subjects were divided into three stages and each mouse was given different dose of the extract. LD50 calculated by mean of mortality lowest dose and non mortality highest dose then the result classified into the degree of acute toxicity. The study showed there was no mortality in all groups of the experimental subject. LD50 of unripe Carica papaya L. aqueous extract was found >5000 mg/kg BW and classified as Practically Non Toxic (PNT) substance.
Full text article
References
Aravind G, Bhowmik D, Duraivel S, Harish G. 2013. Traditional and medicinal uses of Carica papaya. Journal of Medicinal Plants Studies. 1(1):7–15.
BPOM. 2014. Pedoman uji toksisitas nonklinik secara in vivo. Peraturan Kepala Badan Pengawas Obat dan Makanan Republik Indonesia No. 7 Tahun 2014.
Chinedu E, Arome D, Ameh FS. 2013. New recomended method for determining acute toxicity in animal models. Toxicology International. 20(3):224–6.
Dewoto HR. 2007. Pengembangan obat tradisional Indonesia menjadi fitofarmaka. Maj Kedokt Indon. 57(7):205–11.
Figueiredo AC, Barroso JG, Pedro LG, Scheffer JJC. 2008. Factors affecting secondary metabolite production in plants: volatile components and essential oils. Flavour and Fragrance Journal. 23(4):213–26.
Kharisma Y, Ariyoga A, Sastramihardja HS. 2011. Efek ekstrak air buah pepaya (Carica papaya L.) muda terhadap gambaran histologi kelenjar mamma mencit laktasi. MKB.43(4):160–5.
Hodgson E. 2004. Introduction to toxicology. In: Hodgson E, penyunting. A textbook of modern toxicology. Edisi ke-3. New Jersey (AS):Wiley & Sons, Inc.
Iwuagwu M, Chukwuka KS, Uka UN. 2013. Evaluation of nutritional components of Carica papaya L. at different stages of ripening. Journal of Pharmaceutical and Biological Sciences. 6(4):13–6.
Lila MA, Raskin I. 2005. Health-related interactions of phytochemical. Journal of Food Science. 70(1):21–7.
Paramveer D, Chancal M, Paresh M, Rani A, Nema RK. 2010. Effective alternative methods of LD50 help to save number of experimental animals. Journal of Chemical and Pharmaceutical Research. 2(6):450–3.
Oduola T, Adeniyi FAA, Ogunyemi EO, Bello IS, Idowu TO, Subair, HG. 2007. Toxicity studies on an unripe Carica papaya aqueous extract: biochemical and haematological effects in wistar albino rats. Journal of Medicinal Plants Research. 1(1):1–4.
Ramakrishna A, Ravishankar GA. 2011. Influence of abiotic stress signals on secondary metabolites in plants. Plant Signaling and Behavior Landes Bioscience. 6(11):1720–31.
Authors
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. Copyright @2017. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted non-commercial used, distribution and reproduction in any medium