The Isolation and Identification of Active Compound of Dendrophthoe praelonga (Blume) Miq. Extract Against Breast Cancer Cells (MCF-7)
Article Sidebar
-
Anticancer, Antioxidant, Petai plant leaves
Abstract
Petai mistletoe (Dendrophthoe praelonga (Blume) Miq) is semi-parasitic plant. Parasite plant has been used traditionally in Indonesia to prevent or treat degenerative diseases, such as cancer. The purpose of this study was to indentify anticancer compound of petai mistletoe leaves extract and to examine its antioxidant activity (based on DPPH method) and cytotoxic activity on breast cancer (MCF-7) cell lines in vitro using Alamar blue method. This research aims to isolating, characterize, determine antioxidant activity and cytotoxic activity. Separation was conducting column chromathography and characterize was conducted using TLC, FT-IR, LC-MS and NMR-1D. From the result obtained, the isolated compound is flavonol quercetine (3,3,7,3’,4’ pethahydroxyflavone) which active antioxidant with IC50 value of 9.4 μg/mL and the value of cytotoxic activity with IC50 750.62 μg/mL
Full text article
References
Artanti N, Firmansyah T, Darmawan A.2012, Bioactivities evaluation of Indonesian mistletoes (Dendrophthoe pentandra (L.) Miq.) leaves extracts. JAPS. 2(1): 24-27.
Dai J, Mumper RJ. 2010. Plant Phenolics: Extraction, Analysis and Their Antioxidant and Anticancer Properties. Molecules. 15(10): 7313-52.
Desai AG, Qazi GN, Ganju RK, El-Tamer M, Singh J, Saxena AK, et al. 2008. Medicinal plants and cancer chemoprevention. Curr Drug Metab. 9(7): 581–591.
Fauzi AN, Nur Azmi MN, Yaacob NS. 2011. Tualang honey induces apoptosis and disrupts the mitochondrial membrane potential of human breast and cervical cancer cell lines. Food and Chemical Toxicology. 49(4 : 871-78
Fulda S, Galuzzi L, Kroemer G. 2010. Targeting mitochondria for cancer therapy. Nature Review. 9(1): 447-461
Gamal, Septananda E. 2013. Potential analysis of cottonwood parasite (Dendropthoe pentandra) stem extract in decreasing of mutant P53 protein expression on cervical cancer cell (HeLa Cells) in vitro. JIMKI. 1(2): 11-15.
Hanahan D, Weinberg RA. 2011. Hallmarks of cancer: the next generation. Cell. 144(5): 646–74.
Isnindar, Wahyuono, S., & Setyowati, E. P. 2011.Isolasi dan identifikasi senyawa antioksidan daun kesemek (diospyros kaki Thunb.) dengan metode DPPH (2,2-Difenil-1 Pikrilhidrazil). Majalah Obat Tradisional. 16(3): 157-164.
Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. 2011. Global cancer statistics. CA Cancer J Clin. 61(2): 69–90.
Ko EY, Moon A. 2015. Natural products for chemoprevention of breast cancer. J Cancer Prev. 20(4): 223–231.
Molyneux, P. 2004. The use of stable free radikal diphenylpicrilhidrazyl (DPPH) for estimating antioxidant activity. Journal Science of Technology. 26(2): 211-9.
N.A.S.N Zainudin and M.D. Sul’ain. 2015. Antiproliferative effect of Dhendrophthoe petandra extract toward human breast adenocarcinoma cell (MCF-7). Jurnal Teknologi. 77(2): 35-39.
Pearce A, Haas M, Viney R, Pearson S-A, Haywood P, Brown C, et al. 2017. Incidence and severity of self-reported chemotherapy side effects in routine care. PLOS ONE. 12(10) : e0184360.
Risky TA, Suyatno. 2014. Aktivitas Antioksidan dan Antikanker Ekstrak Metanol Tumbuhan Paku Adiantum philippensis L. J UNESA Chem. 3(1): 89-94
Sandhar HK, Kumar B, Prasher S, Tiwari P, Salhan M, Sharma P. 2011. A Review of Phytochemistry and Pharmacology of Flavonoid. Inth Pharm Sci. 1(1): 25-41
Siegel RL, Miller KD, Jemal A. 2017. Cancer statistics. CA Cancer J Clin. 67(1): 7–30.
T. Fitrilia, M Bintang, and M Saithri. 2015. Phytochemical Screening and antioxidant activity of clove mistletoe leaf extract (Dendrophthoe petandra (L) Miq). IOSR Journal of Pharmacy. (5) 8: 13-18
Torre LA, Siegel RL, Ward EM, Jemal A. 2016. Global cancer incidence and mortality rates and trends--an Update. Cancer Epidemiol Prev Biomark. 25(1): 16–27.
Wicaksono MH, Permana S. 2013. Potensi fraksi etanol benalu mangga (Dendrophthoe pentandra) sebagai agen anti kanker kolon pada mencit (Mus musculus Balb/c) setelah induksi Dextran Sulvat (DSS) dan Azoxymethane (AOM). Jurnal Biotropika. 1(2): 75-79.
W. Widowati, T. Mozef, C Risdian, H. Ratnawati, S. Tjahjani, and F. Sandra. 2011. The Comparison of Antioxidant and proliferation inhibitor properties of Piper betle L, Catharanthus roseus (L) G.Don ,D.petandra L.,Curcuma manga Val.exctract on T47D cancer line.International Research Journal of Biochemistry and Bioinformatics. 1(2) : 22-28
Authors
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. Copyright @2017. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted non-commercial used, distribution and reproduction in any medium