Bioassay-Guided and In Silico Identification of α-Amylase Inhibitors from Abelmoschus esculentus Fruit
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Abelmoschus esculentus, Antidiabetes, Bioassay-guided, In silico, Inhibitor α-amilase
Abstract
Diabetes mellitus is a metabolic disorder characterized by persistent hyperglycemia. Inhibition of α-amylase is one therapeutic strategy for controlling postprandial blood glucose levels. This study aimed to identify active compounds responsible for α-amylase inhibitory activity in Abelmoschus esculentus fruit using a bioassay-guided and in silico approach. Methanol extract, fractions, and subfractions were evaluated in vitro for α-amylase inhibition. The most active subfraction was characterized by LC–MS and further analyzed by molecular docking and ADMET prediction. Subfraction 2 showed the strongest inhibitory activity, with 74.2634 ± 4.7091% inhibition. LC–MS identified nine compounds, four of which showed favorable binding affinity and interaction patterns with α-amylase: 2-hydroxy-7-methoxycadalene, N-E-coumaroyl tyramine, homovanillic acid, and quercetin. These findings suggest that A. esculentus fruit contains bioactive compounds with potential α-amylase inhibitory activity.
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References
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