Immunostimulatory Effect of Cayratia trifolia L. Domin on Granulocytes and Natural Killer Cells in Tuberculosis-Induced Rats

Muhammad Ilyas Y Ilyas Y; Wa Ode Salma; Mubarak Mubarak; Nasrudin Nasrudin; Parawansah Parawansah; Fadhliyah  Malik; Asriullah  Jabbar; Asniar  Pascayantri; Esti  Badia; Sernita Sernita; Nirwati  Rusli; Lukman La Basy

doi:10.29244/jji.v11i3.433

Muhammad Ilyas Y Ilyas Y⁽¹⁾, Wa Ode Salma⁽²⁾, Mubarak Mubarak⁽³⁾, Nasrudin Nasrudin⁽⁴⁾, Parawansah Parawansah⁽⁵⁾, Fadhliyah Malik⁽⁶⁾, Asriullah Jabbar⁽⁷⁾, Asniar Pascayantri⁽⁸⁾, Esti Badia⁽⁹⁾, Sernita Sernita⁽¹⁰⁾, Nirwati Rusli⁽¹¹⁾, Lukman La Basy⁽¹²⁾

(1) Politeknik Bina Husada Kendari, Jl. Sorumba No. 17, Kendari, Sulawesi Tenggara, 93117,

(2) Department of Nutrition Science, Faculty of Public Health, Universitas Halu Oleo, Kendari, 93232,

(3) Faculty of Medicine, Universitas Halu Oleo, Kendari, 9323,

(4) Department of Pharmacy, Faculty of Pharmacy, Universitas Halu Oleo, Kendari, 93232,

(5) Department of Pharmacy, Faculty of Pharmacy, Universitas Halu Oleo, Kendari, 93232,

(6) Department of Pharmacy, Faculty of Pharmacy, Universitas Halu Oleo, Kendari, 93232,

(7) Department of Pharmacy, Faculty of Pharmacy, Universitas Halu Oleo, Kendari, 93232,

(8) Department of Pharmacy, Faculty of Pharmacy, Universitas Halu Oleo, Kendari, 93232,

(9) Politeknik Bina Husada Kendari, Jl. Sorumba No. 17, Kendari, Sulawesi Tenggara, 93117,

(10) Politeknik Bina Husada Kendari, Jl. Sorumba No. 17, Kendari, Sulawesi Tenggara, 93117,

(11) Politeknik Bina Husada Kendari, Jl. Sorumba No. 17, Kendari, Sulawesi Tenggara, 93117,

(12) Pharmacy Study Program, STIKES Maluku Husada, Ambon, 97128

https://doi.org/10.29244/jji.v11i3.433

Issue
Vol. 11 No. 3 (2026): Jurnal Jamu Indonesia (In Progress)

Submitted
August 6, 2025

Accepted
March 12, 2026

Published
May 29, 2026

Keywords:

Cayratia trifolia, Immunomodulator, Granulocytes, Natural killer cells, Tuberculosis

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Abstract

Pulmonary tuberculosis (TB) remains a major public health problem in Indonesia and a leading cause of death from infectious diseases, partly because of impaired host immunity. Galing (Cayratia trifolia L. Domin) has been reported to exert immunomodulatory effects in bacterial infections. This study evaluated the immunostimulatory effect of galing leaf and stem extracts on granulocytes and natural killer (NK) cells in a TB-induced rat model. Leaf and stem extracts were administered orally to 28 Wistar rats at doses of 400 and 500 mg/kg body weight for seven days, followed by induction with TB ESAT-6 antigen. Granulocyte counts were measured using a hematology analyzer, whereas NK cells were quantified by ELISA. Galing extract significantly increased granulocyte and NK cell expression compared with the negative control (p<0.05). These findings indicate that galing extract has potential as an immunostimulatory agent in pulmonary TB by enhancing granulocyte and NK cell responses.

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Authors

Muhammad Ilyas Y Ilyas Y

Politeknik Bina Husada Kendari, Jl. Sorumba No. 17, Kendari, Sulawesi Tenggara, 93117

ilyasyusufmuhammad.apt@gmail.com (Primary Contact)

Wa Ode Salma

Department of Nutrition Science, Faculty of Public Health, Universitas Halu Oleo, Kendari, 93232

Mubarak Mubarak

Faculty of Medicine, Universitas Halu Oleo, Kendari, 9323

Nasrudin Nasrudin